Abscisic Acid Priming Creates Alkaline Tolerance in Alfalfa Seedlings (Medicago sativa L.)
نویسندگان
چکیده
Soil alkalization triggers ion toxicity and osmotic alkaline (high pH) stresses in plants, damaging their growth productivity. Therefore, we investigated whether priming with abscisic acid (ABA) increases the tolerance of alfalfa seedlings to stress, then examined underlying molecular mechanisms. Alfalfa were pretreated ABA (10 μM) for 16 h subjected stress using a 15 mM Na2CO3 solution (pH 10.87). Compared control, pretreatment significantly alleviated leaf damage improved fresh weight, water content, survival rate under conditions. Abscisic reduced accumulation reactive oxygen species (ROS), increased activities antioxidant enzymes superoxide dismutase (SOD) peroxidase (POD), maintained higher ratios K+/Na+, Ca2+/Na+, Mg2+/Na+, proline. In addition, upregulated expression genes involved proline biosynthesis (P5CS) sequestration Na+ vacuoles (NHX1 AVP) by maintaining homeostasis ROS metal ions upregulating osmoprotection tolerance-related genes.
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ژورنال
عنوان ژورنال: Agriculture
سال: 2021
ISSN: ['2077-0472']
DOI: https://doi.org/10.3390/agriculture11070608